IBA GmbH
IBA Lifesciences MagStrep 'Type3' Strep-Tactin Beads 5% Suspension
MagStrep 'type3' Strep-Tactin beads 5% suspension are magnetic beads to purify biotinylated proteins.
IBA Lifesciences Strep-Tactin™ Sepharose™ Resin
Strep-Tactin™ Sepharose™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences MagStrep Strep-Tactin™ XT Beads
MagStrep Strep-Tactin™ XT beads are magnetic beads (5% suspension) to purify Strep-tag™ II or Twin-Strep-tag™ fusion proteins.
IBA Lifesciences Strep-Tactin™ Conjugate
Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins
IBA Lifesciences Strep-Tactin™XT 4Flow™ Resin
Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
IBA Lifesciences Twin-Strep-tag™ Capture Kit
The Twin-Strep-tag™ Capture Kit can be used for reversible capture of Twin-Strep-tag™ fusion proteins for SPR analyses
IBA Lifesciences MEXi-293E Cells
IBA Lifesciences GmbH MEXi-293E Cells are an efficient and cost effective mammalian protein expression system.
IBA Lifesciences MEXi-CM
IBA Lifesciences GmbH MEXi-CM Culture Medium is an efficient and cost effective mammalian protein expression system.
IBA Lifesciences MEXi-TM
IBA Lifesciences GmbH MEXi-TM Transfection Medium is an efficient and cost effective mammalian protein expression system.
IBA Lifesciences Competent E. coli TOP10
Competent E. coli TOP10 are chemically competent cells for plasmid propagation
IBA Lifesciences Strep-Tactin™ 4Flow™ Cartridge
Strep-Tactin™ 4Flow™ cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
Twin-Strep-tag™/Strep-Tactin™XT
The purification system with the highest affinity – one tag for expression, purification, detection and immobilization
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Immobilization with near covalent binding affinity
Twin-Strep-tag™ Capture Kit for SPR experiments »
- High affinity in low pM range enables long term measurements (T1/2 = 13 h)
- Chip can be easily regenerated
High affinity meets high capacity!
Strep-Tactin™XT Superflow™ high capacity »
- Higher protein yields compared to all other Strep-Tactin™ resins
- Dynamic binding capacity of ~15 mg protein per ml resin
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
Try our new high affinity Strep-tag™ system
Starter Kit consists of:
- Strep-Tactin™XT Superflow™ gravity flow column
- Buffer W (Strep-Tactin™/Strep-Tactin™XT Wash Buffer)
- Buffer BXT (Strep-Tactin™XT Elution Buffer with Biotin)
- Strep-Tactin™ conjugated with HRP (for Western blot detection)
- Short protocol
Traceless Affinity Cell Selection
Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection
- High purity, yield and viability
- Label-free cells
- Directly from whole blood
- No centrifugation
Magnetic Cell Selection
- Fully reversible
- No stimulation of cells after cell separation
- Optimal preservation of cell effector function
- Improved viability of cells
Cell Stimulation & Expansion
- Completely reversible reagents
- Detachment from the cells at any given point in time
- Adjustable CD3 : CD28 ratio
- Non-magnetic and bead-free
Peptides for T cell stimulation »
- Purity > 80 %
- Endotoxin free
Fluorescent Cell Staining
Detection of antigen-specific T cells with MHC I Streptamers™ »
Detection of receptor-specific T cells with Fab Streptamers™ »
- Fully reversible
- High specificity, sensitivity and reproducibility
- No leftover of staining reagents on the cells after FACS sorting
- Optimal preservation of cell effector function
- Improved viability of cells
Cloning & Transfection
More than 150 expression vectors »
- Variety of affinity tags
- Expression hosts: E. coli, mammalia, insect cells, yeast
- StarGate cloning system
Mammalian Expression
Optimized for efficient protein expression and Strep-tag™ purification in mammalian cells.
Protein Purification
Strep-tag™ system from the original manufacturer
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Detection
For Western blot analysis, immunofluorescence, FACS and ELISA »
- Strep-Tactin™ conjugates for Strep-tag™II/Twin-Strep-tag™ proteins
- Monoclonal antibodies against Strep-tag™II/Twin-Strep-tag™
Immobilisation
Near covalent binding affinity – Immobilisation by Strep-Tactin™XT:Twin-Strep-tag™ for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore)
- High affinity in low pM range enables measurements over a long time
- Minimal non-specific binding
- Minimal coefficients of variation (cv)
- Chip for SPR experiments can be easily regenerated
Immunoprecipitation
Selector resins based on high-affinity single-domain antibodies (sdAb)
- High capacity
- Minimal unspecific binding
DNA
RNA
Standard DNA oligonucleotides
- Long range up to 200 bases*
- Large scale oligonucleotides
- 3’-, 5’- or internally modified
Random RNA, dsRNA, siRNA, miRNA
- Long range up to 100 bases*
- 3’-, 5’- or internally modified
- 2‘-modified: -O-Me, -F, -NH2
- Nucleoside Triphosphates
- RNA and RNA/DNA hybrid
- Dinucleotides
*depending on sequence and scale
Fluorescence Labels
Non-Fluorescence Labels
E.g. for FRET, qPCR, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami
- For dual and multiply labeled DNA and RNA
- Excellent high quality
- More than 200 labels to choose from
- Quenchers such as BBQ and BHQ™
- Completely new dye combinations available
For DNA microarrays, surface binding and hybridization experiments, cross-linking and crystallography studies and more
- More than 80 internal, 5’- and 3’- DNA and RNA modifications
Backbone Modifications
Aptamers
- Phosphorothioates
Custom synthesis of DNA and RNA aptamers
- High affinity
- Low immunogenicity
Cell Selection & Expansion
- Application Note FABian Lymphocyte Isolation
- Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
- Application Note Combining MHC-I and CD8+ Fab Streptamer reagents
- Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
- Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Custom Oligos & Predefined DNA/RNA
About IBA GmbH
Solutions For Life Sciences
IBA GmbH provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.